Re: Fluorescent stain for leukocytes

Posted by simon walker (BI)-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Bleaching-of-background-tp7578651p7578667.html

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We routinely use the lipophilic dye Cell Mask Orange (Invitrogen) to label neutrophils in chemotaxis assays.  Our exps are not usually longer than half an hour, but we see no loss of signal during this time.
Simon

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of B. Prabhakar Pandian
Sent: 16 July 2012 21:18
To: [hidden email]
Subject: Fluorescent stain for leukocytes

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Hello,
            Can someone suggest a stable fluorescent stain for live cell imaging of leukocytes upto 4 hours.  We are using Rhodamine but the signal goes away rapidly.
We keep the lamp off during the non-acquisition time, hence photo-bleaching is not the main issue.

Thanks,

-Prabhakar
The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT Registered Charity No. 1053902.
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