Posted by
Tim Feinstein-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/best-FRET-pair-2012-update-tp7578698p7578700.html
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Hi Martin,
From what I understand CFP/YFP and its close variants such as cerulean, venus, etc remain almost freakishly effective compared with most alternatives. If there exists a green/orange or orange/red pair with similar efficiency and lack of unwanted artifacts (e.g., photoconverson) then it is news to me. About why that is, maybe it has to do with the odd emission spectrum of CFP. Or perhaps it involves some quantum whatsit that the folks at CERN can figure out now that they have found their Higgs boson.
cheers,
TF
Timothy Feinstein, PhD
Postdoctoral Fellow
Laboratory for GPCR Biology
Dept. of Pharmacology & Chemical Biology
University of Pittsburgh, School of Medicine
BST W1301, 200 Lothrop St.
Pittsburgh, PA 15261
On Jul 20, 2012, at 6:15 AM, Martin Offterdinger wrote:
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> Dear all,
>
> This question has been asked in the past already. But the color palette of
> XFPs has increased substantially in the meantime.
> Which combination of FPs is considered the best FRET pair right now?
> Maybe one should also seperate between acceptor bleaching, sensitized
> emission (ie filter fret) and FLIM, since the demands are quite different
> for the three methods.
> Any input is very welcome!
>
>
> Martin