Posted by
Periasamy, Ammasi (ap3t) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/best-FRET-pair-2012-update-tp7578698p7578701.html
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Hello
If you look at the literature, majority of the time Cerulean and Venus is used as a FRET pair. The CFP is not a good donor for lifetime since it provides double exponential decays.
Cerulean3 and mTFP also used as a donor and Venus as an acceptor. Venus and tdTomato is another good FRET pair too. Please see some of our publications for various FRET pairs
1. Sun, Y., Wallrabe, H. Booker, C., Day, R.N. and Periasamy, A. (2010) Three-color spectral FRET microscopy localizes three interacting proteins in living cells. Biophysical J. Vol. 99, 1274-1283.
2. Sun, Y. Booker, C.F., Kumari, S., Day, R.N., Davdison, M. and Periasamy, A. (2009)
Characterization of an Orange Acceptor Fluorescent Protein for Sensitized Spectral FRET Microscopy using a White Light Laser. J. Biomed. Opt. 14(5), 054009 (pp1-11).
3.Day, R.N., Booker, C. and Periasamy, A. (2008) The Characterization of an improved donor fluorescent protein for Förster resonance energy transfer microscopy. J. Biomed. Opt. 13: 031203 (pp1-9).
Hope this helps
ammasi
Dr. Ammasi Periasamy
Professor & Center Director
W.M. Keck Center for Cellular Imaging (KCCI)
Biology, University of Virginia
B005, Physical Life Sciences Building (PLSB)
90, Geldard Drive, Charlottesville, VA 22904
(Campus Mail - P.O. Box 400328)
Voice: 434-243-7602 (Office); 982-4869 (lab)
Fax:434-982-5210; Email:
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12th Annual Workshop on FRET Microscopy, March 11-16, 2013
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-----Original Message-----
From: Confocal Microscopy List [mailto:
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Sent: Friday, July 20, 2012 6:15 AM
To:
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Subject: best FRET pair _2012 update
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Dear all,
This question has been asked in the past already. But the color palette of XFPs has increased substantially in the meantime.
Which combination of FPs is considered the best FRET pair right now?
Maybe one should also seperate between acceptor bleaching, sensitized emission (ie filter fret) and FLIM, since the demands are quite different for the three methods.
Any input is very welcome!
Martin