CFP and GFP proteins

Posted by Ramana Sidhaye on
URL: http://confocal-microscopy-list.275.s1.nabble.com/CFP-and-GFP-proteins-tp7578754.html

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Hi,
  One of my collaborators has a mouse endogenously expressing 2 proteins (both are expressed in the same cells), one coupled to CFP and the other to GFP. I am curious about the spectrum- how does one best evaluate both proteins in the same sample without concerns about cross-reactivity.

Thanks,
Ramana SIdhaye