http://confocal-microscopy-list.275.s1.nabble.com/CFP-and-GFP-proteins-tp7578754p7578758.html
GFP using a YFP filter set. If you're using filters for your imaging, you
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> Separating the two proteins should not be too difficult if you have the
> right filter sets, or if you have a spectral microscope system. If you
> check the spectra (e.g. invitrogen spectra viewer which you can Google),
> you will see that GFP emission starts at ~ 500 nm, so a narrow CFP filter
> should give litttle or no GFP signal. Conversely, if you excite GFP at
> around 488 nm, there should be little or no CFP excitation. Your
> institution probably has a Microscopy Core facility, so the best is to talk
> to them... I'm sure they can point you in the right direction.
>
> --
> Julio Vazquez
> Fred Hutchinson Cancer Research Center
> Seattle, WA 98109-1024
>
>
>
http://www.fhcrc.org>
>
> On Aug 1, 2012, at 12:55 PM, Ramana Sidhaye wrote:
>
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> > Hi,
> > One of my collaborators has a mouse endogenously expressing 2 proteins
> (both are expressed in the same cells), one coupled to CFP and the other to
> GFP. I am curious about the spectrum- how does one best evaluate both
> proteins in the same sample without concerns about cross-reactivity.
> >
> > Thanks,
> > Ramana SIdhaye
>