Imaging collagen with circularly polarized light

Posted by Michael Abanto on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Cosmic-ray-particles-centenary-The-Chase-2012-tp7578803p7578804.html

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Hi,
    Does anybody have a good protocol for imaging collagen in embedded tissue, stained with picrosirius red? I had no problem imaging collagen with linear polarization, but I wanted to try circular and found it difficult.
    I have an Olympus BX61 and a Nikon A1, as well as demo lambda plates for both. Do I need an analyzer specific for circular polarization? And do I need a linear polarizer before the circular - and how would you set that up on the Olympus?
   Any tips or help on the set-up would be appreciated,
   Mike