Re: Imaging collagen with circularly polarized light

Posted by Alberto Diaspro on Aug 09, 2012; 1:01pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/Cosmic-ray-particles-centenary-The-Chase-2012-tp7578803p7578805.html

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maybe this set-up could help
Diaspro, A., Bertolotto, M., Vergani, L., & Nicolini, C. (1991). Polarized light scattering of nucleosomes and polynucleosomes-in situ and in vitro studies. IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 38(7), 670–678.


Il giorno 09/ago/2012, alle ore 14:15, Michael Abanto <[hidden email]> ha scritto:

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> Hi,
>    Does anybody have a good protocol for imaging collagen in embedded tissue, stained with picrosirius red? I had no problem imaging collagen with linear polarization, but I wanted to try circular and found it difficult.
>    I have an Olympus BX61 and a Nikon A1, as well as demo lambda plates for both. Do I need an analyzer specific for circular polarization? And do I need a linear polarizer before the circular - and how would you set that up on the Olympus?
>   Any tips or help on the set-up would be appreciated,
>   Mike