Posted by
Guy Cox-2 on
Aug 10, 2012; 3:32pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/Cosmic-ray-particles-centenary-The-Chase-2012-tp7578803p7578813.html
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The signal will always be weaker with CP light. But it will no longer be orientation dependent. See Pu Xu, Eleanor Kable, Colin J. R. Sheppard, and Guy Cox, 2010 A quasi-crystal model of collagen microstructure based on SHG microscopy. Chinese Optics Letters 8, 213-216
Do be aware that your quarter wave plate must be quarter wave for your excitation wavelength - a visible light one will not work if you are using SH / 2P excitation. (Lambda/4 at 900 nm = lambda/2 at 450nm). The analyser is less critical.
Guy
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Alberto Diaspro
Sent: Thursday, 9 August 2012 11:01 PM
To:
[hidden email]
Subject: Re: Imaging collagen with circularly polarized light
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maybe this set-up could help
Diaspro, A., Bertolotto, M., Vergani, L., & Nicolini, C. (1991). Polarized light scattering of nucleosomes and polynucleosomes-in situ and in vitro studies. IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 38(7), 670-678.
Il giorno 09/ago/2012, alle ore 14:15, Michael Abanto <
[hidden email]> ha scritto:
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>
> Hi,
> Does anybody have a good protocol for imaging collagen in embedded tissue, stained with picrosirius red? I had no problem imaging collagen with linear polarization, but I wanted to try circular and found it difficult.
> I have an Olympus BX61 and a Nikon A1, as well as demo lambda plates for both. Do I need an analyzer specific for circular polarization? And do I need a linear polarizer before the circular - and how would you set that up on the Olympus?
> Any tips or help on the set-up would be appreciated,
> Mike