simple test to compare confocals

Posted by Arvydas Matiukas on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Cosmic-ray-particles-centenary-The-Chase-2012-tp7578803p7578820.html

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Dear List,
 
I am looking for  simple and quick test (and sample) to compare
acquisition across different confocals. Specifically, to our LSM510
there was recently added Nikon C2 , and users want to know how
they compare by few practical parameters, e.g. signal sensitivity,
spectral bleedthrough, bleaching, and  maybe resolution  .
 
I am aware of papers by Zucker, Pawley, Cole that describe detail
evaluation of confocal performance, and even recently measured
some PSFs. However, I am looking for just simple and quick
test that would allow direct visual comparison of images (simple
analysis like getting intensity histogram is fine) acquired on different
confocals.
 
Please share your thoughts and/or experience.
 
Thank you in advance,
Arvydas
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Arvydas Matiukas, Ph.D.
Director of Confocal&Two-Photon Core
Department of Pharmacology
SUNY Upstate Medical University
766 Irving Ave., WH 3167
Syracuse, NY 13210
tel.: [phone]
fax: [phone]
email: [hidden email]