Re: processing and Z cropping tiled Leica images

Posted by Thomas Trusk on
URL: http://confocal-microscopy-list.275.s1.nabble.com/processing-and-Z-cropping-tiled-Leica-images-tp7578835p7578850.html

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I've been experimenting quite a bit with the latest incarnation of Leica's
stitching routine in LAS, and it is MUCH improved.  The automatic mode does
use content as a guide, so blank or sparsely populated images present a
problem.  If you turn AUTO off, the stitching is faster and edge-to-edge.

I should note that Leica offers a new option, that I have not had a chance to
see, that supposedly pre-maps focus over the entire area to be stitched.  I've
been told this function would allow one to scan only areas where regions of
interest exist, preventing the blank image problem and saving lots of storage
space.

Thomas C. Trusk, PhD
Director, Josh Spruill Imaging Facility
Regenerative Medicine and Cell Biology
Medical University of South Carolina
Charleston, SC  29425
http://mmi.musc.edu