Re: Background fluorescence problem

Posted by Matt Kofron on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Background-fluorescence-problem-tp7579031p7579041.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Have you tried a narrow YFP filter? A 500/20 excitation filter should still
excite GFP but shift away from the flavin peak. You will see less GFP, but
it might improve S/N.