Re: Sealing cover slips

Posted by Knecht, David on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Sealing-cover-slips-tp7579054p7579055.html

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We seal them for live cell work with Valap- equal parts vaseline, paraffin wax, lanolin heated on a hot plate at 100C and applied with a fine art brush.  Dave

On Sep 18, 2012, at 4:42 PM, Mike Tighe wrote:

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Does anybody know of a non toxic seal for coverslips? I am looking at live-dead stained bacteria (hoping to see them die) but I think that my agarose is shinking due to evaporation over time. I am worried that nail polish or vacuum grease will affect my results.



As usual, thanks for any help!

Mike

Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)