Re: Sealing cover slips

Posted by Guy Cox-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Sealing-cover-slips-tp7579054p7579057.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I always use wax in this situation.  Of course it's easy for me since the EM lab always has a dish of molten wax at hand (it's used for sealing the water-bath on glass microtome knives.)  But it completely avoids the toxic effects of the solvents in nail polish.  

                                                                                       Guy

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Mike Tighe
Sent: Wednesday, 19 September 2012 6:43 AM
To: [hidden email]
Subject: Sealing cover slips

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Does anybody know of a non toxic seal for coverslips? I am looking at live-dead stained bacteria (hoping to see them die) but I think that my agarose is shinking due to evaporation over time. I am worried that nail polish or vacuum grease will affect my results.



As usual, thanks for any help!

Mike