Posted by
David Baddeley on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Sealing-cover-slips-tp7579054p7579060.html
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Something we've used on occasion is 2-part dental silicone (the same stuff that is used for making impression moulds) - the exact name and supplier will vary depending on where you are. You mix the parts (I tend to use a small weighing dish to mix in), and apply a bead round the edge of the coverslip (I usually use a pippette tip for this). I've mostly used it for fixed cells, but have heard it touted as being good for live cells (if you can put it in your mouth, it can't be particularly toxic) . It also sets slightly faster than nail-polish, which is helpful if you are under time pressure.
cheers,
David
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From: Chris Booth <
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To:
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Sent: Wednesday, 19 September 2012 8:58 AM
Subject: Re: Sealing cover slips
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This is a non-commercial post. In our lab we use an organic coverslip sealer
from the company Biotium called CoverGrip. It comes in the same kind of
container as nail polish, with a little brush attached to the lid for ease of use.
I've had great luck using this product and the price is extremely fair for a RTU
reagent, especially if you don't have the ingredients to make a custom cocktail.
Definition from the site: CoverGrip Coverslip Sealant is made with d-limonene, a
natural, environmentally-friendly solvent with a pleasant citrus aroma, and dries
to form a clear, hard, durable seal along the edges of the coverslip.
http://biotium.com/product/product_types/search/price_and_info.asp?
item=23005