Posted by
Naomi Book on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Sealing-cover-slips-tp7579054p7579061.html
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We use Sylgard, you can probably find it a neurobiology lab that does patch
clam they use it to seal the patch electrodes. Its non toxic and easy to
use.
Naomi Book
-----Original Message-----
From: Confocal Microscopy List [mailto:
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Behalf Of David Baddeley
Sent: Wednesday, September 19, 2012 12:36 AM
To:
[hidden email]
Subject: Re: Sealing cover slips
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Something we've used on occasion is 2-part dental silicone (the same stuff
that is used for making impression moulds) - the exact name and supplier
will vary depending on where you are. You mix the parts (I tend to use a
small weighing dish to mix in), and apply a bead round the edge of the
coverslip (I usually use a pippette tip for this). I've mostly used it for
fixed cells, but have heard it touted as being good for live cells (if you
can put it in your mouth, it can't be particularly toxic) . It also sets
slightly faster than nail-polish, which is helpful if you are under time
pressure.
cheers,
David
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From: Chris Booth <
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To:
[hidden email]
Sent: Wednesday, 19 September 2012 8:58 AM
Subject: Re: Sealing cover slips
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This is a non-commercial post. In our lab we use an organic coverslip sealer
from the company Biotium called CoverGrip. It comes in the same kind of
container as nail polish, with a little brush attached to the lid for ease
of use.
I've had great luck using this product and the price is extremely fair for a
RTU
reagent, especially if you don't have the ingredients to make a custom
cocktail.
Definition from the site: CoverGrip Coverslip Sealant is made with
d-limonene, a
natural, environmentally-friendly solvent with a pleasant citrus aroma, and
dries
to form a clear, hard, durable seal along the edges of the coverslip.
http://biotium.com/product/product_types/search/price_and_info.asp?
item=23005