Re: Sealing cover slips

Posted by Littlejohn, George on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Sealing-cover-slips-tp7579054p7579062.html

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Hi Mike,

I like using Carolina Observation Gel (http://www.carolina.com/product/life+science/microscope+slides/protist+slides/carolina+observation+gel.do, no commercial interest).

It is mouldable and non-toxic polydimethylsiloxane, which can be made into custom gaskets to seal slides (I cut them to shape with a needle). It allows gas exchange, but minimises evaporation.

Best wishes,
George

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Dr. George Littlejohn
School of Biosciences,
University of Exeter,
Halpin Laboratory,
Geoffrey Pope Building,
Stocker Road,
EX4 4QD, UK
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On 18 Sep 2012, at 21:44, "Mike Tighe" <[hidden email]> wrote:

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> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> Does anybody know of a non toxic seal for coverslips? I am looking at live-dead stained bacteria (hoping to see them die) but I think that my agarose is shinking due to evaporation over time. I am worried that nail polish or vacuum grease will affect my results.
>
>
>
> As usual, thanks for any help!
>
> Mike