Posted by
Andrew York on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deconvolution-advice-tp7579090.html
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Hello, I'm looking for advice and information about deconvolution,
especially from those with first-hand experience.
Traditionally, one of the processing steps in structured illumination
microscopy is deconvolution. For our SIM, we decided to use an open-source
solution:
https://sites.google.com/site/piotrwendykier/software/deconvolution/paralleliterativedeconvolutionThis seemed like a nice tradeoff between reinventing the wheel with our own
deconvolution code, and subjecting ourselves to a 'black box' closed-source
solution. However, we've recently tried out the Huygens deconvolution
software, and the results seem quite promising, possibly an improvement
over other methods we've tried. I like good images, but I don't like black
boxes, and I like to understand my data processing.
1. Is the exact algorithm used in Huygens transparently documented
anywhere? I spent a few hours searching today, but if it's out there, I
missed it.
2. Is there a clear winner for deconvolution algorithms? What should I be
using?
3. Are there other deconvolution software packages I should consider?
Ideally I'm looking for software based on clearly-documented algorithms.
Thanks for the help.
-Andrew York
NIH/NIBIB