http://confocal-microscopy-list.275.s1.nabble.com/Deconvolution-advice-tp7579090p7579091.html
well thought-out. I don't know too much about the exact algorithms, but
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> Hello, I'm looking for advice and information about deconvolution,
> especially from those with first-hand experience.
>
> Traditionally, one of the processing steps in structured illumination
> microscopy is deconvolution. For our SIM, we decided to use an open-source
> solution:
>
>
https://sites.google.com/site/piotrwendykier/software/deconvolution/paralleliterativedeconvolution>
> This seemed like a nice tradeoff between reinventing the wheel with our own
> deconvolution code, and subjecting ourselves to a 'black box' closed-source
> solution. However, we've recently tried out the Huygens deconvolution
> software, and the results seem quite promising, possibly an improvement
> over other methods we've tried. I like good images, but I don't like black
> boxes, and I like to understand my data processing.
>
> 1. Is the exact algorithm used in Huygens transparently documented
> anywhere? I spent a few hours searching today, but if it's out there, I
> missed it.
>
> 2. Is there a clear winner for deconvolution algorithms? What should I be
> using?
>
> 3. Are there other deconvolution software packages I should consider?
> Ideally I'm looking for software based on clearly-documented algorithms.
>
> Thanks for the help.
>
> -Andrew York
> NIH/NIBIB
>
Joel B. Sheffield, Ph.D