http://confocal-microscopy-list.275.s1.nabble.com/Deconvolution-advice-tp7579090p7579093.html
Model, etal, Journal of Microscopy, Vol. 241, Pt 1 2011, pp. 94–100
I believe the Huygens algorithm has been published. Just email them,
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> Hi Andrew,
>
> I don't have first-hand experience but I remember reading this two-part
> comparison of different deconvolution softwares, including commercial and
> open-source ones, in GIT Imaging & Microscopy by the Sage's group at EPFL
> (direct links to pdfs):
>
>
http://bigwww.epfl.ch/publications/griffa1001.pdf>
http://bigwww.epfl.ch/publications/griffa1002.pdf>
> Hope this helps,
>
> Christophe
>
> On Fri, Sep 28, 2012 at 10:33 PM, Andrew York <
>
[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>> *****
>>
>> Hello, I'm looking for advice and information about deconvolution,
>> especially from those with first-hand experience.
>>
>> Traditionally, one of the processing steps in structured illumination
>> microscopy is deconvolution. For our SIM, we decided to use an open-source
>> solution:
>>
>>
https://sites.google.com/site/piotrwendykier/software/deconvolution/paralleliterativedeconvolution>>
>> This seemed like a nice tradeoff between reinventing the wheel with our own
>> deconvolution code, and subjecting ourselves to a 'black box' closed-source
>> solution. However, we've recently tried out the Huygens deconvolution
>> software, and the results seem quite promising, possibly an improvement
>> over other methods we've tried. I like good images, but I don't like black
>> boxes, and I like to understand my data processing.
>>
>> 1. Is the exact algorithm used in Huygens transparently documented
>> anywhere? I spent a few hours searching today, but if it's out there, I
>> missed it.
>>
>> 2. Is there a clear winner for deconvolution algorithms? What should I be
>> using?
>>
>> 3. Are there other deconvolution software packages I should consider?
>> Ideally I'm looking for software based on clearly-documented algorithms.
>>
>> Thanks for the help.
>>
>> -Andrew York
>> NIH/NIBIB
>>