Oil vs water objectives

Posted by Gabriel Lapointe-4 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Oil-vs-water-objectives-tp7579114.html

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Hi,

I have a user who insist that using a 1,27NA water immersion objective is
brighter and would give better images than using a 1,4NA oil immersion. I
understand that deeper into the media that would be true. But, in that
particular case, we are talking about imaging GFP at less than 100 micron
away with a spinning disk.

So, I was wondering at which distance from the coverslips do we start
seeing benefits of using a water immersion objective over an oil objective
in aqueous media.

Thanks for your help.

Sincerely
*Gabriel Lapointe, M.Sc.*
Lab Manager / Microscopy Specialist
Concordia University, Biology Department
7141 Sherbrooke St. West SP 534
Montréal QC H4B 1R6 Canada
[hidden email]
cmac.concordia.ca
http://gabriellapointe.ca