Re: Oil vs water objectives

Posted by mcammer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Oil-vs-water-objectives-tp7579114p7579115.html


This is the type of question that can result in a big theoretical discussion, but in my experience dealing with a wide variety of (mostly) biological material prepared in many different ways, trying each optic for an empirical comparison is the way to go.  

________________________________________________________
Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Gabriel Lapointe
Sent: Thursday, October 04, 2012 9:15 AM
Subject: Oil vs water objectives

Hi,

I have a user who insist that using a 1,27NA water immersion objective is brighter and would give better images than using a 1,4NA oil immersion. I understand that deeper into the media that would be true. But, in that particular case, we are talking about imaging GFP at less than 100 micron away with a spinning disk.

So, I was wondering at which distance from the coverslips do we start seeing benefits of using a water immersion objective over an oil objective in aqueous media.

Thanks for your help.

Sincerely
*Gabriel Lapointe, M.Sc.*
Lab Manager / Microscopy Specialist
Concordia University, Biology Department
7141 Sherbrooke St. West SP 534
Montréal QC H4B 1R6 Canada
[hidden email]
cmac.concordia.ca
http://gabriellapointe.ca