Re: Oil vs water objectives

Posted by Mark Cannell-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Oil-vs-water-objectives-tp7579114p7579118.html

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Do they have the same magnification?

Mark
On 4/10/2012, at 2:59 PM, "Cammer, Michael" <[hidden email]> wrote:

>
> This is the type of question that can result in a big theoretical discussion, but in my experience dealing with a wide variety of (mostly) biological material prepared in many different ways, trying each optic for an empirical comparison is the way to go.  
>
> ________________________________________________________
> Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine
> Lab: [phone]  Cell: [phone]
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Gabriel Lapointe
> Sent: Thursday, October 04, 2012 9:15 AM
> Subject: Oil vs water objectives
>
> Hi,
>
> I have a user who insist that using a 1,27NA water immersion objective is brighter and would give better images than using a 1,4NA oil immersion. I understand that deeper into the media that would be true. But, in that particular case, we are talking about imaging GFP at less than 100 micron away with a spinning disk.
>
> So, I was wondering at which distance from the coverslips do we start seeing benefits of using a water immersion objective over an oil objective in aqueous media.
>
> Thanks for your help.
>
> Sincerely
> *Gabriel Lapointe, M.Sc.*
> Lab Manager / Microscopy Specialist
> Concordia University, Biology Department
> 7141 Sherbrooke St. West SP 534
> Montréal QC H4B 1R6 Canada
> [hidden email]
> cmac.concordia.ca
> http://gabriellapointe.ca
>
>

Mark  B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology&  Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

[hidden email]