Posted by
Guy Cox-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Oil-vs-water-objectives-tp7579114p7579128.html
That's not really true. If you cannot validate the method, how can you trust your image of your sample?
Guy
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Cammer, Michael
Sent: Friday, 5 October 2012 2:20 AM
To:
[hidden email]
Subject: Re: Oil vs water objectives
Also, while people have generated various test samples of collagen and latex and acrylic and fat globules and glass beads and carrageen gum and dissolved Scotch tape glue etc., the only test sample that really answers the question is the sample itself.
________________________________________________________
Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine
Lab: (212) 263-3208 Cell: (914) 309-3270
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Steffen Dietzel
Sent: Thursday, October 04, 2012 10:30 AM
To:
[hidden email]
Subject: Re: Oil vs water objectives
So we are back at Michael's comment: You've got to test it. Beads attached to the coverslip and the slide (various preparations with various distances between the two) give good test objects.
Steffen