Definite Focus for multi position time-lapse imaging

Posted by luidale on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Definite-Focus-for-multi-position-time-lapse-imaging-tp7579162.html

Hello

We have Zeiss LSM 710 and I want to use it for multi position time-lapse imaging preferable with Definite Focus. Problem is that the Definite Focus switches itself automatically off when one is doing multi-position imaging.

Could the usage of some Macros solve this issue. Otherways said is there any macro available controlling DefFoc during multi position time-lapse imaging (eg. activating DF at each position). For example MultiTime. But as far as I understand MultiTime is meant for using AutoFocus which is to slow for me and I have to pay for it also.

Hannes