Question about deconvolution

Posted by lechristophe on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Question-about-deconvolution-tp7579203.html

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Hi folks,

I have a long-standing question regarding deconvolution (as processing
widefield or confocal images to reassign light from where it originated
using a PSF).

Is there a theoretical limit to the resolution one could obtain using
deconvolution? Is is theoretically possible to "break" the diffraction
limit with deconvolution? That is, to get under the classical 200x200x600nm
spot? I think it is not the case, but then why would you deconvolve
widefield or confocal images? What do you gain by doing so on a system that
is reasonably close to its theoretical capabilities in terms of optical
performances?

Thanks for your help,

Christophe

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Christophe Leterrier
Researcher
Axonal Domains Architecture Team
CRN2M CNRS UMR 7286
Aix Marseille University, France