Re: TIRF depth calibration

Posted by Michal Opas on Nov 01, 2012; 9:28pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/TIRF-depth-calibration-tp5769934p7579264.html

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Dear Listers,

My search of archives leads me nowhere hence this question:

we need to mount spherical cell aggregates (mouse EBs) circa 300 µm in
diameter in a mountant that would let them wiggle while focused up and
down and, importantly, prevent coverslip from moving and shearing them
to shreds. Our commercial mountant (DAKO) does not cure. Nail polish
remedies this a bit but I'd hope for an improvement with a mountant that
would gel (solidify) in a decent time.

Thank you very much in advance!

Michal*
<http://www.utoronto.ca/mocell>*