Re: TIRF depth calibration

Posted by Sarang Kulkarni on
URL: http://confocal-microscopy-list.275.s1.nabble.com/TIRF-depth-calibration-tp5769934p7579266.html

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Just a guess, but you could try a very low percentage low melt agarose spread very thinly.  This should set in a decent amount of time.  Not sure about the wiggle room that you desire.

Sarang Kulkarni

Sent from my iPhone

On 2012-11-01, at 5:33 PM, "Michal Opas" <[hidden email]> wrote:

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> Dear Listers,
>
> My search of archives leads me nowhere hence this question:
>
> we need to mount spherical cell aggregates (mouse EBs) circa 300 µm in diameter in a mountant that would let them wiggle while focused up and down and, importantly, prevent coverslip from moving and shearing them to shreds. Our commercial mountant (DAKO) does not cure. Nail polish remedies this a bit but I'd hope for an improvement with a mountant that would gel (solidify) in a decent time.
>
> Thank you very much in advance!
>
> Michal*
> <http://www.utoronto.ca/mocell>*