Re: Cy3/Cy5 registration on fluoview 1000

Posted by Claire Brown on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Cy3-Cy5-registration-on-fluoview-1000-tp7579371p7579384.html

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The ABRF light microscopy research group (www.abrf.org/lmrg) did a study on
channel registration and the details are published in Microscopy and
Microanalysis including how to accurately measure this shift:

http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8332637

I am not surprised it is high when using cy5 many lenses are not well
corrected out in the far red. If you follow the protocol and take high
resolution z-stacks of tetraspek beads you should be able to precisely
calculate the shift between Cy3 and Cy5 for your lens.

Good luck!

Claire