Posted by
Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Cy3-Cy5-registration-on-fluoview-1000-tp7579371p7579390.html
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On 29.11.2012 18:08, Jean-Pierre CLAMME wrote:
> Also what software are you using to do a good Z registration ? We have
> imaris and I found imaris unable to do subpixel registration (At my
> previous place I was using Slidebook to do subpixel registration).
The Sync Measure tool in ImageJ or Fiji was designed with that
application in mind. You need stacks of mentioned multicolorbeads (or
other signals that show up in the channels in questions) and the tool
will evaluate the gravity centers in the various color channels in
parallel.
For a short description see
http://rsbweb.nih.gov/ij/plugins/sync-windows.htmlSteffen
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy
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