Posted by
John Oreopoulos on
URL: http://confocal-microscopy-list.275.s1.nabble.com/History-lesson-tp7579399p7579400.html
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Andrew,
See these publications:
Cremer, C., et al., Superresolution imaging of biological nanostructures by spectral precision distance microscopy. Biotechnology Journal, 2011. 6(9): p. 1037-1051.
Huber, O., et al., Localization microscopy (spdm) reveals clustered formations of p-glycoprotein in a human blood-brain barrier model. Plos One, 2012. 7(9).
Kaufmann, R., et al., Visualization and quantitative analysis of reconstituted tight junctions using localization microscopy. Plos One, 2012. 7(2).
My understanding is that this is yet another form of super-resolution localization microscopy. Here's a clipping from the first paper:
"Originally [10], in the scheme to explain the SPDM principle, the different spectral signatures were indicated using color. In later publications [22, 24], the color assignment was deliberately omitted to make it clear that differences in the fluorescence emission spectrum (called color) are just one of the many ways to realize a spectral signature difference useful for superresolution. In the original definition of ‘spectral signature’ [10, 14], any photophysical property of the fluorescent point emitters was included that allowed the independent registration (‘optical isolation’) of the respective diffraction patterns. In particular, it was recognized that the SPDM principle as described was applicable to various kinds of far-field fluorescence microscopy, including in particular focused, structured, and homogeneous illumination."
Cheers,
John Oreopoulos
Research Assistant
Spectral Applied Research
Richmond Hill, Ontario
Canada
www.spectral.ca
On 2012-12-04, at 8:20 AM, Andrew York wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> I recently stumbled across a Wikipedia article that caught my eye:
>
http://en.wikipedia.org/wiki/Christoph_Cremer>
> I'm not familiar with the work described in this article, but it seems
> astounding.
> In particular:
> Widefield localization microscopy of whole cells in two minutes, with 10 nm
> resolution
> Structured illumination microscopy with 40 nm resolution.
>
> Have I been negligent in my citations? Why haven't I heard of this before?