seeking suggestions on new sCMOS camera

Posted by Zulin Yu on
URL: http://confocal-microscopy-list.275.s1.nabble.com/seeking-suggestions-on-new-sCMOS-camera-tp7579501.html

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Dear All,
    I am writing to ask your suggestions about sCMOS camera on spinning disk
system. Recently, I  got a demo (Hamamatsu Flash 4.0) camera on our spinning
disk system (PerkinElmer Ultraview) and I am a little bit disappointed about its
performance  - it is a decent camera, but not as good as I expected.
    Based on the company's brochure, Flash 4.0 SNR exceeds that of EM-CCDs at
about 6 photon/pixel
(http://sales.hamamatsu.com/assets/pdf/hpspdf/e_flash4.pdf); several research
paper also claimed that the latest sCMOS is as good as EMCCD with more than 15
photons (http://www.ncbi.nlm.nih.gov/pubmed?term=EMCCD%20sCMOS).
However, we still got a much higher SNR (double) on EMCCD even we have more
than 50 photons/pixel.  Did anybody have experience with the high end sCMOS
camera and compared it with back-thinned EMCCD? I appreciate any input, Zulin