Posted by
Zdenek Svindrych on
URL: http://confocal-microscopy-list.275.s1.nabble.com/seeking-suggestions-on-new-sCMOS-camera-tp7579501p7579503.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy*****
Hi Zulin,
So the CMOS is better than your EMCCD? Then go for it!
We are using Andor Neo, it is faster and has more pixels than EMCCDs. Also
the image looks OK. We are using the CMOS on SIM, where the light level is
not such an issue. We are consitering it also for STORM where it should be
comparable to EMCCD.
We also have a spinning disc with back-thinned EMCCD an ad I recall the
camera already saturates with (almost) minimum laser level at 30fps with 200
x gain (provided the sample is well stained, of course). So there is also a
lot of light and I assume the CMOS should do the job.
As to the noise of your EMCCD, I'm not sure how you calibrated the photon
flux. I am only able to calculate the photon flux from the poisson noise of
an image series, so the noise then always appears 'correct' with respect to
the photon count. So your photon calibration might be wrong.
If you suspect your camera has exrta noise, try to plot the standard
deviation of your pixels versus mean intensity for a broad range of
intensities. Then you should be able to identify the extra noise. You may
also try to record the same intensity distribution without EM gain and with
the other (non-EM, slow) port.
Cheers,
zdenek
---------- Původní zpráva ----------
Od: Zulin Yu <
[hidden email]>
Datum: 16. 1. 2013
Předmět: seeking suggestions on new sCMOS camera
"*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy*****
Dear All,
I am writing to ask your suggestions about sCMOS camera on spinning disk
system. Recently, I got a demo (Hamamatsu Flash 4.0) camera on our spinning
disk system (PerkinElmer Ultraview) and I am a little bit disappointed about
its
performance - it is a decent camera, but not as good as I expected.
Based on the company's brochure, Flash 4.0 SNR exceeds that of EM-CCDs at
about 6 photon/pixel
(
http://sales.hamamatsu.com/assets/pdf/hpspdf/e_flash4.pdf); several
research
paper also claimed that the latest sCMOS is as good as EMCCD with more than
15
photons (
http://www.ncbi.nlm.nih.gov/pubmed?term=EMCCD%20sCMOS).
However, we still got a much higher SNR (double) on EMCCD even we have more
than 50 photons/pixel. Did anybody have experience with the high end sCMOS
camera and compared it with back-thinned EMCCD? I appreciate any input,
Zulin"