Posted by
Arne Seitz on
URL: http://confocal-microscopy-list.275.s1.nabble.com/seeking-suggestions-on-new-sCMOS-camera-tp7579501p7579505.html
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Comparing cameras is always very difficult and the outcome will strongly depend on the application. My personal strategy is always to rather rely on own data than what was written in product brochures or papers. Thus if you get better images with an EM CCD camera this is the camera of choice.
I tested an PCO.edge sCMOS camera (no commercial interest) on the new Yokogawa W1 spinning disk head and I was really impressed. For me the main advantage of this type of camera is the large field of view in combination with the high acquisition speed. Also the small pixel is a plus as it allows imaging under nyquist conditions (or even doing oversampling) with low magnification objectives having a high NA. Nevertheless at low light application I prefer using a back illuminated EM CCD camera.
And on a conventional spinning disk system it is at least questionable whether a sCMOS camera is the best camera, as large areas of the chip remain unused.
Regards
Arne
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:
[hidden email]] On Behalf Of Zulin Yu
> Sent: mercredi 16 janvier 2013 05:46
> To:
[hidden email]
> Subject: seeking suggestions on new sCMOS camera
>
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>
> Dear All,
> I am writing to ask your suggestions about sCMOS camera on spinning disk
> system. Recently, I got a demo (Hamamatsu Flash 4.0) camera on our
> spinning disk system (PerkinElmer Ultraview) and I am a little bit
> disappointed about its performance - it is a decent camera, but not as good
> as I expected.
> Based on the company's brochure, Flash 4.0 SNR exceeds that of EM-CCDs
> at about 6 photon/pixel
> (
http://sales.hamamatsu.com/assets/pdf/hpspdf/e_flash4.pdf); several
> research paper also claimed that the latest sCMOS is as good as EMCCD with
> more than 15 photons
> (
http://www.ncbi.nlm.nih.gov/pubmed?term=EMCCD%20sCMOS).
> However, we still got a much higher SNR (double) on EMCCD even we have
> more than 50 photons/pixel. Did anybody have experience with the high
> end sCMOS camera and compared it with back-thinned EMCCD? I appreciate
> any input, Zulin