Posted by
Tim Feinstein-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Imaging-bacteria-help-tp7579528p7579530.html
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Hi Shane,
When studying toxic algae, we would often affix them to lysine-coated slides for antibody staining by spotting 50-100 ul of fixed cells in a square or circle drawn with a wax pencil, and then place the slides in a special holder and spin them in a large swinging-bucket benchtop centrifuge. You may have to spin bacteria longer or faster but the same principle should apply. Cannot help with your first question.
cheers,
TF
Timothy Feinstein, PhD
Visiting Research Associate
Laboratory for GPCR Biology
Dept. of Pharmacology & Chemical Biology
University of Pittsburgh, School of Medicine
BST W1301, 200 Lothrop St.
Pittsburgh, PA 15261
On Jan 21, 2013, at 11:27 AM, Shane van Breda wrote:
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Hi everyone,
>
> I have two questions about imaging bacteria (specifically Tuberculosis or any
> Mycobacterium).
>
> 1.) I am interested in the mycolic acids of the bacteria. Does anyone know of
> a specific fluorescent dye (with specific wavelengths) or any anti - mycolic
> acid antibodies for immunolabelling?
>
> 2.) What would be the best way to fix the bacteria to slides from broth? They
> need to fixed and dead since they are pathogenic. But I would like to view
> whole cells and not sections from resin.
>
> Thanks very much for your input.
>
> Shane