Posted by
Rob Palmer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Imaging-bacteria-help-tp7579528p7579544.html
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Shane - what exactly are you trying to do? It is unlikely that you
will be able to differentiate between different mycolic acid
structures using anything other than very well characterized
antibodies together with immunoTEM. If you are trying to distinguish
cells that have mycolic acids from those that do not, there are
probably easier ways unrelated to mycolic acids. What do you want to
do with your bacteria from broth? Simply drying washed cells onto a
slide coated with polylysine ought to do the trick. I think you
understand that these cells are very small compared to most eukaryotic
cells and, depending on what exactly you want to see, EM or image-
processing of confocal/digital-decon images may be the way to go. You
can contact me off-list if you'd like to discuss in detail.
On Jan 21, 2013, at 11:27 AM, Shane van Breda wrote:
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>
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>
> Hi everyone,
>
> I have two questions about imaging bacteria (specifically
> Tuberculosis or any
> Mycobacterium).
>
> 1.) I am interested in the mycolic acids of the bacteria. Does
> anyone know of
> a specific fluorescent dye (with specific wavelengths) or any anti -
> mycolic
> acid antibodies for immunolabelling?
>
> 2.) What would be the best way to fix the bacteria to slides from
> broth? They
> need to fixed and dead since they are pathogenic. But I would like
> to view
> whole cells and not sections from resin.
>
> Thanks very much for your input.
>
> Shane
Robert J. Palmer Jr., Ph.D.
Microbial Receptors Unit
Laboratory of Cell and Developmental Biology
Natl Inst Dental Craniofacial Res - Natl Insts Health
Bldg 30, Room 310
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396