FISH and Immunofluorescence

Posted by Casal Moreno, Carmen on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Refraction-and-Dispersion-phase-contrast-tp7579532p7579555.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi everyone,

I am Carmen Casal, and I am working in the Microscopy Unit of Cancer Epigenetics and Biology Program in Barcelona, Spain.
I am wondering if some of you have done an Immunofluorescence in combination with FISH. I know that there are some papers describing the combined method but my question is that if I have to detect an hybrid structure formed with DNA and RNA (RLoop) and for this motive I can't denaturalize the structure because I am sure that it will broke with the treatment, and also I have to do an Immunofluorescence to detect the gene where this kind of structure is located, how can I do it?

Thank you very much.
Best regards,
Carmen.


Carmen Casal Moreno, PhD
Microscopy Unit
Programa d'Epigenètica i Biologia del Càncer (PEBC)
Fundació Institut d'Investigació Biomèdica de Bellvitge (IDIBELL) 
Av.Gran Via de L'Hospitalet, 199 - 203
08908 L'Hospitalet de Llobregat
Barcelona
932607500 ext 3246
[hidden email]