Ratiometric FRET on Fluoview

Posted by Jean-Pierre CLAMME-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Ratiometric-FRET-on-Fluoview-tp7579865.html

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Hello,

I saw a paper about Ratiometric FRET (Roszik, Cytometer 2009) mentioning
line by line acquisition of the 3 images (IDA, IDD, IAA). The authors
mentioned the use of a LSM 510.

I don't know the LSM510, but I have a fluoview 1000 and the only way I can
see how to do that is using the "virual Channels" . However that would be
image by image and not line by line.

Does anyone has done ratiometric FRET on the fluoview and what method did
you use ?

Thank you and Best regards,

JP


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Jean-Pierre CLAMME, PhD
Chief Scientist
Nitto Denko Technical
501 Via Del Monte
Oceanside, CA 92058
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