Re: Ratiometric FRET on Fluoview sorry

Posted by Jean-Pierre CLAMME-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Ratiometric-FRET-on-Fluoview-tp7579865p7579866.html

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Sorry I meant

"Has anyone done ratiometric FRET on the fluoview and what method did
you use ? "




Confocal Microscopy List <[hidden email]> wrote on
03/04/2013 02:43:44 PM:

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> Hello,

> I saw a paper about Ratiometric FRET (Roszik, Cytometer 2009) mentioning
> line by line acquisition of the 3 images (IDA, IDD, IAA). The authors
> mentioned the use of a LSM 510.

> I don't know the LSM510, but I have a fluoview 1000 and the only way I
can
> see how to do that is using the "virual Channels" . However that would
be
> image by image and not line by line.

> Does anyone has done ratiometric FRET on the fluoview and what method
did
> you use ?

> Thank you and Best regards,

> JP

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