Posted by
Andreas Bruckbauer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Ratiometric-FRET-on-Fluoview-tp7579865p7579872.html
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I have not used it for FRET, but we have a sequential tick box at the bottom of the window with the channel parameters. When ticked we can select "frame by frame" or "line by line" aquisition and how the channels will be grouped together.
best wishes
Andreas
-----Original Message-----
From: Jean-Pierre CLAMME <
[hidden email]>
To: CONFOCALMICROSCOPY <
[hidden email]>
Sent: Mon, 4 Mar 2013 22:44
Subject: Ratiometric FRET on Fluoview
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Hello,
I saw a paper about Ratiometric FRET (Roszik, Cytometer 2009) mentioning
line by line acquisition of the 3 images (IDA, IDD, IAA). The authors
mentioned the use of a LSM 510.
I don't know the LSM510, but I have a fluoview 1000 and the only way I can
see how to do that is using the "virual Channels" . However that would be
image by image and not line by line.
Does anyone has done ratiometric FRET on the fluoview and what method did
you use ?
Thank you and Best regards,
JP
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Jean-Pierre CLAMME, PhD
Chief Scientist
Nitto Denko Technical
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