Re: antibodies for immunofluorescence

Posted by Carol Heckman on
URL: http://confocal-microscopy-list.275.s1.nabble.com/antibodies-for-immunofluorescence-tp7579900p7579904.html

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Michal-
I would think you need to consider whether it is a monoclonal or polyclonal antibody.  If you have a blocking antibody, such as the peptide the antibody is raised against, it would help show that it is a specific identification.  The monoclonal antibody only attaches a single epitope.
Carol Heckman

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From: Confocal Microscopy List [[hidden email]] On Behalf Of Michał Majkowski [[hidden email]]
Sent: Friday, March 08, 2013 3:36 AM
To: [hidden email]
Subject: antibodies for immunofluorescence

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Dear All,
I do not know if this is a good place to address my problem as it
regards immunofluorescence. The problem is: we have in the lab
antibodies dedicated to Western Blot (according to manufacturer; Abcam).
We used them to IF and we obtained some nice images. What do you think
about such data? Should it be confirmed by IgG dedicated to IF?   Thank
you for help.
Best,
Michal