Re: Stage Top Incubator

Posted by Gabriel Lapointe-4 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Cheap-scope-components-tp7579847p7579978.html

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We have a Tokai and a Chamlide stage incubators. I strongly recommend the
water column  over the Tokai moat. The CO2 air mix is still very dry when
it gets into the tokai and it get humidified from both the moat and the
samples. There is also issues when it's time to clean up. The chamber is
permanently fixed to the controller, so you basically have to pour the
water into a beaker while standing over your microscope.

As for gas, i haven't seen a system that mesure the amount of gas inside
the chamber itself. An air mixer will mix it for you from a 100% CO2
cylindre but most of the time I saw people buying the pre-mixed gas. Our
chamlide have a very smoth thick wall compare to the cheap plastic of the
Tokai. This has an impact on our gas consumption by about 60-75 %.

Sincerely,
*Gabriel Lapointe, M.Sc.*
Lab Manager / Microscopy Specialist
Concordia University, Biology Department
7141 Sherbrooke St. West SP 534
Montréal QC H4B 1R6 Canada
Lab : [phone] x5988
Office : [phone] x3008
Fax : [phone]
Cell : [phone]
[hidden email]
cmac.concordia.ca
http://gabriellapointe.ca


2013/3/13 B. Prabhakar Pandian <[hidden email]>

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>
> Hello All,
>                   We are looking to buy a stage top incubator. However,
> one of the questions which has come up is whether the gases are running
> continuously to maintain levels since it is open near the objective.
> Are there gaskets to seal around slides/petri dishes so that a feedback on
> the levels can be provided and the gas flow can be upregulated or
> downregulated depending on the need.   Finally, most of them
> have a bottle of water for humidifying the chamber. Only Tokai Hit has
> tray with water in the insert itself. Any thoughts on the pros/cons is
> appreciated.
>
> Any other feedback to help us make a sound decision will be great.
>
> Thanks,
>
> -Prabhakar
>