Re: Nunc Lab-Tek problem

Posted by Myriam Gastard on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nunc-Lab-Tek-problem-tp7579967p7579979.html

Hi Michael,

I am glad you didn't get any interferences while using your slides. I have tested extensively some of their multi-chamber slides I got huge reflection not only from the plastic bottom but also from the well walls. It is to be noted that it was tested in confocal and using STED and the high power CW laser could have contributed to that obviously.
They do say that while their bottom is a #1.5, it is not a standard glass. I had some discussions with Ibidi and they were then quite surprised by the results. Still, I have not yet tried a non-standard bottom and would be glad to try them. Nevertheless, if their bottom cover has not triggered problems in your experiments, then maybe you can try 1 or 2 wells slides which have a big cell adhesion surface (no commercial interest!)
ibidi Standard Bottom

The ibidi Standard Bottom, which is our standard surface, is a hydrophilic, foil-like coverslip that forms the bottom of most ibidi μ-Slides, μ-Dishes, and μ-Plates. The Standard Bottom meets all optical requirements for various imaging techniques, such as phase contrast,confocal fluorescence, or two-photon microscopy.


Best,
Myriam

Myriam Gastard, PhD
From my iPad.

On Mar 13, 2013, at 3:23 PM, "Cammer, Michael" <[hidden email]> wrote:

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>
> Ibidi makes many versions of chambers.  Some do have glass bottoms.  We routinely use two designs in our lab with #1.5 coverslips on the bottom, but we want the larger open chambers for other experiments.
>
> Regards,
>
> Michael
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Myriam Gastard
> Sent: Wednesday, March 13, 2013 2:10 PM
> To: [hidden email]
> Subject: Re: Nunc Lab-Tek problem
>
> Hi Michael,
>
> Be aware that Ibidi slides bottom are not made of glass and will interfere with a lot of reflection during imaging.
> Hope this will help,
>
> Myriam
>
> Myriam Gastard, PhD
> From my iPad.
>
> On Mar 13, 2013, at 12:08 PM, "Deepak Saini" <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hi Michael,
>>
>> Look at ibidi they have quite a few designs and may be some can fit
>> your requirements.
>>
>> No commercial interest.
>>
>> Deepak
>>
>>
>> On Wed, Mar 13, 2013 at 8:47 PM, Cammer, Michael
>> <[hidden email]
>>> wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Hi.
>>>
>>> For many years we used Nunc Lab-Tek chambered coverglasses. They were
>>> great.
>>>
>>> However, they recently began shipping a different design with the
>>> same product number. The chambers no longer fit in our microscope
>>> stages inserts. Also, the volume changed.
>>> see http://www.flickr.com/photos/mcammer/sets/72157632984171809/
>>>
>>> While we can adjust to the volume change, the plastic tab at the end
>>> of the chamber is a big problem.
>>>
>>> I thought of having people cut the tab off before doing experiments,
>>> but this would produce a lot of plastic dust (and we'd have to buy a
>>> suitable saw or dremel).
>>>
>>> Does anybody know of an alternative manufacturer or if the old style
>>> chambers are still available from somewhere?
>>>
>>> Thanks!
>>>
>>> Michael
>>>
>>>
>>>
>>>
>>> ________________________________________________________
>>> Michael Cammer, Assistant Research Scientist Skirball Institute of
>>> Biomolecular Medicine
>>> Lab: [phone]  Cell: [phone]
>>>
>>
>>
>>
>> --
>> ----------------------------------------------------------------------
>> ------------------------------
>> Deepak K Saini, PhD
>> Assistant Professor
>> Department of Molecular Reproduction, Development and Genetics Indian
>> Institute of Science (IISc) Bangalore, India.
>> [hidden email]
>> [hidden email]
>> Tel: [phone]
>> ----------------------------------------------------------------------
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>> <[hidden email]>