Posted by
Mike Buchin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Cheap-scope-components-tp7579847p7579995.html
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No commercial interest, just FYI:
We just saw a new stage (15 in the field so far) top from In Vivo Scientific
yesterday. It can hold a full well plate format and I believe it has most
of the optimal features being discussed in this email chain. Barney Boyce
sent us a full up manual but I am pretty sure that it would be blocked as an
attachment if I tried to send it. Request the manual for the
controller(ECU) and stage top environment(STEV) from Invivo:
[hidden email]
Good luck,
Mike
Michael Buchin
Stanford Photonics, Inc.
Ph: 650-969-5991
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On
Behalf Of Julio Vazquez
Sent: Thursday, March 14, 2013 12:46 PM
To:
[hidden email]
Subject: Re: Stage Top Incubator
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Prabhakar:
Just one more comment:
As was pointed out by Gabriel, the Chamlide incubator does not monitor CO2
inside the chamber, as far as I know (no evidence of any sensor in there).
It probably just mixes CO2 and Air and delivers that to the chamber. The
chamber is small and engineered pretty tight though, and we use very little
CO2 (2-3 sbps, or "small bubbles per second"). I don't know if the system
from Pathology Devices monitors CO2 or not.
On the other hand, In Vivo scientific does have an incubator system where
CO2 is monitored right inside the incubation chamber:
http://www.invivoscientific.com/Peripherals.htmI believe this system though is designed to work with the larger type of
incubator (that wraps around the scope) for temperature control. There is a
second chamber that fits inside and holds the specimen. The CO2 probe goes
in there. I think the key is to have a chamber that is well sealed,
otherwise there is too much exchange of gas with the outside and CO2 control
will be erratic. You may want to check the exact specs with the vendor if
interested.
--
Julio Vazquez,
Fred Hutchinson Cancer Research Center
On Mar 13, 2013, at 10:32 AM, B. Prabhakar Pandian wrote:
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>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Hello All,
> We are looking to buy a stage top incubator. However, one
of the questions which has come up is whether the gases are running
continuously to maintain levels since it is open near the objective.
> Are there gaskets to seal around slides/petri dishes so that a feedback on
the levels can be provided and the gas flow can be upregulated or
downregulated depending on the need. Finally, most of them
> have a bottle of water for humidifying the chamber. Only Tokai Hit has
tray with water in the insert itself. Any thoughts on the pros/cons is
appreciated.
>
> Any other feedback to help us make a sound decision will be great.
>
> Thanks,
>
> -Prabhakar