Blocking in immunocytochemsitry - ImageIT FX Enhancer?

Posted by lechristophe on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Blocking-in-immunocytochemsitry-ImageIT-FX-Enhancer-tp7580055.html

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Hi,

Not a confocal question, but a lot of people are doing immuno here so I
thought I'd ask. We are currently using gelatin as the main blocker in our
imunocytochemistry protocol. I was wondering if we could get better
staining (less background / less non specific labeling) by switching to
something else and found Life Technologie's ImageIT FX Enhancer (
http://products.invitrogen.com/ivgn/product/I36933). Does someone have
experience with this, and is the added cost justified? I try to stay clear
of proprietary formulas but sometimes (see Prolong Gold) a "secret" product
does work better.

Thanks for the advices,

Christophe

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Christophe Leterrier
Researcher
Axonal Domains Architecture Team
CRN2M CNRS UMR 7286
Aix Marseille University, France