Posted by
Steve Bagley on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Slide-scanner-tp7580065p7580080.html
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Hi Sue
we have two system in my lab the 3D-histech Scan which has been operating for five years now and the Lecia scn400 for two years.
After the latest upgrade the 3D-Histech system keeps the focus well in both imaging modes, the Leica system also keeps focus well and operates at a reasonable speed. We initally had problems with both systems but now on both the operating software seems solid. The 3D-Histech and Leica free software allows for export of grabs of the data at screen resolution.
For analysis we run the software through Definiens Tissue Studio (
http://tissuestudio.definiens.com ) which gave us greater measurement capabilities than the instrument supplied software.
You might want to look at the Perkinelmer Vectra (
http://www.perkinelmer.co.uk/Catalog/Category/ID/Vectra ) as spectrophotometric imaging the tissue would allow greater multiplexing in both brightfield and fluorescence mode, and help to remove autofluorescence. We are considering this at the moment as we wish to throw a huge number of labelling regimes onto our TMAs.
Hope this helps
Steve
Steve Bagley
Head of Advanced Imaging and Flow Cytometry | Paterson Institute for Cancer Research | Cancer Research UK | University of Manchester | Wilmslow Road | Manchester | UK | M20 4BX | www:
http://goo.gl/R828QOn 26 Mar 2013, at 05:07, CONFOCALMICROSCOPY automatic digest system <
[hidden email]<mailto:
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From: Sue Penrhyn-Lowe <
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Subject: Slide scanner
Date: 25 March 2013 16:33:26 GMT
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I am looking to buy a new virtual slide scanner with both Fluorescent and
bright field capabilities. What are your thoughts on the leica SCN400F?
Thank-you
sue
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