Posted by
Glen MacDonald-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/xyzt-image-tp7580102p7580107.html
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I believe Kees was referring the Grouped Z-Project in Fiji/ImageJ, not something in your confocal software. Import your Leica file into Fiji with the LOCI plugin. The Grouped Z-Project will create a new stack in which each frame is the z-projection of the corresponding time point. There are several routes to save that as an AVI from Fiji/ImageJ. It is advisable to also archive the projection movie in an uncompressed format in case you want to edit it in the future. In fact, you can add timestamps and annotations in Fiji. Please refer to documentation:
http://imagej.nih.gov/ij/docs/guide/index.htmlRegards,
Glen
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Cellular Morphology Core
Center on Human Development and Disability
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
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On Apr 3, 2013, at 3:44 AM, Manish Kumar <
[hidden email]> wrote:
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>
> Dear Kees,
> Thanks for the reply. I have tried the option. This works with the confocal
> software, but didnt work when i export it to .avi .
>
> Rgds
> Manish
>
>
> On Wed, Apr 3, 2013 at 3:45 PM, Straatman, Kees R. (Dr.) <
>
[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>>
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>>
>> Dear Manish,
>>
>> You can use Image > Stacks > Tools > Grouped Z-project...
>>
>> Best wishes
>>
>> Kees
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:
[hidden email]]
>> On Behalf Of Manish Kumar
>> Sent: 03 April 2013 10:51
>> To:
[hidden email]
>> Subject: xyzt image
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
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>>
>> Hello everyone,
>>
>> Is there any way to convert XYZT image to xyt. format.
>>
>> I have done live cell imaging with xyzt format and and I want to export
>> the same file to XYT.avi format. That means I want to compile the z stack
>> so that I can get the .avi image in xyt format.
>> I am using Leica SP5 confocal misroscope.
>>
>> Regards
>> Manish Kumar
>> Imaging facility Manager
>> IGIB,
>> New Delhi
>>