Re: colocalization between confocal and two-photon: voxel matching

Posted by Pascal Weber on
URL: http://confocal-microscopy-list.275.s1.nabble.com/xyzt-image-tp7580102p7580118.html

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In fact for colocalization signals, DAPI and FITC, just use the laser 2P. You'll
always have a perfect colocalization. The fluorescence comes as a single dot.
Why would you want to use two different lasers?
For the LSM510  the only way to adjust the filling of the rear lens you have to
ajust the beam size with a lens. I did it and i can modulate the beam size and the
uniformity of the field.

Regards, Pascal