Re: what fluorescent proteins are working best for precision localization microscopy (nanoscopy) - in your and your users hands?

Posted by Rickman, Colin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/what-fluorescent-proteins-are-working-best-for-precision-localization-microscopy-nanoscopy-in-your-a-tp7580157p7580161.html

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Hi

We have used photoactivatable mCherry successfully both for PALM and sptPALM in a couple of published projects.. We started off using photoactivatable GFP which in hindsight is less than optimal due to spontaneous activation and activation induced by the excitation source.

Colin
Dr Colin Rickman
Institute of Biological Chemistry, Biophysics and Bioengineering
School of Engineering and Physical Sciences
Heriot-Watt University
Edinburgh
EH14 4AS

Tel: [phone] (Office)

http://www.ib3.eps.hw.ac.uk
http://www.esric.org


On 10 Apr 2013, at 01:04, George McNamara <[hidden email]> wrote:

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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> Dear confocal listserv,
>
> what fluorescent proteins are working best for precision localization microscopy (nanoscopy) - in your and your users hands?
>
> I know the literature pretty well - what I am looking for is YOUR experiences, ideally good, but if you've been burned, what turned out to be bad.
>
>
> thanks in advance,
>
> George
> p.s. and yes, I will email Michael Davidson directly for his preferences.