Re: Biopsy imaging/holding tools commercial response

Posted by Dan Focht on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Biopsy-imaging-holding-tools-tp7580163p7580169.html

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Bilal


Here is a link to the use of a Corning Snapwell™ turned upside down for upright scopes or right side up with the use of a glass outer ring.

http://www.bioptechs.com/Products/Delta_T/Options/options.html#snapwell

This is typically used for keeping a specimen in contact with a membrane while imaging on a upright scope.
Some people have cut a hole or slit in the membrane to secure their specimen within the tension of the membrane.
You get six of Snapwell membranes in a SBS format plate.
You simply pop off the support holder of the membrane and throw it away and just use the shallow membrane fixture.

The Delta T dish 23mm ID is ideal for the footprint of the Snapwell and fits snugly into a stage adapter.
If you need temperature control the dish provides warmth through a closed loop feedback system. (See Delta T product info page)


Other options:

These links are to a Z axis controlled accessory for the Delta T dish that supports either a coverslip or a membrane immediately above the specimen for inverted microscopes.
The membrane suspension device is used when you need maximum media contact to the specimen and the ability to keep the specimen in place against the coverslip.

http://www.bioptechs.com/Products/Delta_T/Options/options.html#membraneadapter

Here is a similar technique that uses a coverslip instead of a membrane.      

http://www.bioptechs.com/Products/Delta_T/Options/options.html#mediadepth

Here is a link to a product we call a brain slice adapter although it can be used any tissue.
Your specimen is placed on a polycarbonate disk that is lowered into a Delta T Dish with the same type Z axis translator as the above devices where its proximity to the coverslip bottom dish is adjustable.
There is a nylon weight that rest on the top of the specimen to prevent it from floating.

http://www.bioptechs.com/Products/Delta_T/Options/options.html#Brain%20A


Dan




On Apr 10, 2013, at 12:37 PM, Bilal Malik wrote:

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Dear all,



In the process of imaging small (2-4 mm) tissue biopsies under a confocal
microscope with a water-immersion objective, we have had problems holding
the tissue sample down to avoid flotation in water. We have tried using
tweezers with a clip to hold them but are afraid that may damage the biopsy
in some instances, and is not always possible for irregular shaped samples.
Can anyone provide suggestions as to how this problem can be solved either
with a commercial or a custom method?



Thanks so much,

Bilal Malik




Dan Focht
Bioptechs, Inc.
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Butler, PA 16002
www.bioptechs.com
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