Re: what fluorescent proteins are working best for precision localization microscopy (nanoscopy) - in your and your users hands?

Posted by Tom Blanpied on
URL: http://confocal-microscopy-list.275.s1.nabble.com/what-fluorescent-proteins-are-working-best-for-precision-localization-microscopy-nanoscopy-in-your-a-tp7580157p7580174.html

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Hi,
Sorry, I conflated two molecules in my previous post.
In our experience, PA-mCherry provides adequate numbers of molecules, though
isn't as bright or long-lived as mEos2/3.
PA-TagRFP is considerably brighter than PA-mCherry. However, on two or three
different proteins with side-by-side comparisons, it has provided a smaller number
of localized molecules than Eos or Cherry. Thus, longer tracks than PA-mCherry,
but fewer.
Tom